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MoBiTec GmbH(优势品牌)
主营:主营:分子类,蛋白类,抗体类,生化类试剂
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商品详细MoBiTec/分钟™ 细胞质蛋白酶体富集试剂盒/蛋白质组学/PT-040
MoBiTec/分钟™ 细胞质蛋白酶体富集试剂盒/蛋白质组学/PT-040
MoBiTec/分钟™ 细胞质蛋白酶体富集试剂盒/蛋白质组学/PT-040
商品编号: PT-040
品牌: MoBiTec GmbH
市场价: ¥6180.00
美元价: 3708.00
产地: 美国(厂家直采)
公司:
产品分类: 聚合物
公司分类: juhewu
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description:The proteasome is a non-lysosome protein degradation system that requires metabolic energy and polymerization of ubiquitin. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles). Rapid and gentle isolation of proteasome from cells/tissues is essential for the studies of proteasome structure and function. The proteasomes have been found mainly localized in cytosol but also detected in nucleus. Traditionally, ultracentrifugation and affinity isolation are the most common methods for isolation of proteasomes. These methods, though relatively effective, are usually time consuming with low yield. Many affinity-based methods require harsh elution condition that may affect the activity of isolated proteasomes and limit certain downstream applications. To overcome these shortcomings, we have developed a spin-column based technology using a proprietary precipitation buffer for enrichment of proteasome from cytosol by first removing nuclei and majority of organelles followed by preferential precipitation of cytosolic proteasomes. The nuclear proteasomes are excluded with this kit (Nuclear Proteasome Enrichment Kit is available under Cat # PN-041). The protocol is simple and rapid with high yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins. This kit is useful for the studies of proteasome structure and function. The enriched proteasomes can also be used as a starting material for affinity purification.
Order #:PT-040
Unit Size:20 Tests
Supplier:Invent Biotechnologies
Restrictions:Only available in Germany and selected European countries.
Shipping:Ambient
Storage:4 °C
Subcategory:Cell Fractionation
More information:Go to webpage
品牌介绍
MoBiTec GmbH是一家私人控股公司,由Stephan Diekmann教授于1987年创立。与Max-Planck学会的密切关系以及德国领先的研究中心之一哥廷根的所在地促进了与某些研究中心的密切合作。德国最具创造力的科学家。此外,MoBiTec一直与欧洲和北美的科学家广泛合作,包括法国巴斯德研究所和荷兰Vrije大学的科学家。通过将许多研究人员的精彩创意商业化,MoBiTec能够提供一系列独特的高质量产品和简化的协议,使实验室生活更轻松,并为实际研究留出更多时间。 MoBiTec的产品包括e。 G。用于体内检测蛋白质 - 蛋白质或DNA-蛋白质相互作用的双杂交系统,用于筛选特定肽配体的噬菌粒展示系统和选择具有特异性结合特性的新蛋白质,方便的LamBDa PS基因组文库用于克隆的多功能DNA载体,表达和分析靶基因,有效表达和纯化重组蛋白的试剂盒,细胞转染试剂和细胞培养工具,固定化和可溶性酶,许多用于基因组学和蛋白质组学研究的产品(例如用于PCR,核酸和蛋白质纯化和分析),众多抗体,细胞因子,生长因子和重组蛋白,优质荧光试剂和试剂盒(如蛋白标记试剂盒,标记抗体,细胞染色试剂,柱和凝胶电泳附件)。 与其自有产品线并行,MoBiTec分销德国多家国际公司的产品,包括荧光探针和研究化学品(AnaSpec,TEFLabs),量子点,各种DNA纯化试剂盒和DNA聚合酶(EdgeBio),诊断工具(ZJ BioTech) ),凝胶电泳配件(Amresco,Clare Chemical),糖生物学产品(Dextra Laboratories,Sumitomo),细胞转染试剂(Mirus)以及抗体,蛋白质,底物,精细化学品和分子和细胞生物学试剂盒 - 特别是病毒学,免疫学,神经生物学,细胞凋亡,信号转导,细胞增殖,细胞毒性和癌症研究(MBLI,AG Scientific,Amresco,AdarBiotech,AnaSpec,Echelon)。 MoBiTec提供全面的服务,从清晰完整的产品文档开始,为客户提供个性化的建议和技术服务。 MoBiTec产品通过MoBiTec的总部在德国分销,在其他国家由分销商分销。此外,MoBiTec不断寻求新产品和可销售的许可技术,因此对科学合作非常感兴趣,以开发创新的产品理念。